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. 2023 Oct 2;133(19):e162731. doi: 10.1172/JCI162731

Figure 8. Cholesterol affects binding of MS rAbs to PLP1-expressing cells.

Figure 8

(A) Representative images of ON#34 rAb binding to PLP1-transfected CHOK1 cells following 30-minute treatment with vehicle (RPMI), 5 mM BMCD, or 5 mM ΒMCD preloaded with cholesterol (CHOL). Scale bars: 20 µm. (B) Quantitation (mean ± SEM) of background-corrected ratios of MS rAb to PLPAA3 signal normalized to RPMI control binding. Data are represented as ratios of replicate images obtained from more than 3 independent transfection experiments. Significance was determined using ANOVA with Dunnett’s correction for multiple comparisons. **P < 0.01; *** P < 0.001; **** P < 0.0001.