Figure 6. Expression of Glp1r in Lepr neurons mediates food intake suppression by liraglutide in male mice.

(A) Generation of Glp1r^LSL/LSL (KO) and Lepr^Cre; Glp1r^LSL/LSL (Re^Lepr) mice to test the sufficiency of Glp1r expression in Lepr cells for food intake suppression by liraglutide. (B–D) Mice were treated with vehicle for 1 day and then treated with liraglutide (400 μg/d, i.p.) for 3 days (days 0–2) and monitored for food intake and body weight. Daily food intake (B), cumulative food intake over the 3-day treatment period (C), and change in body weight over the 3-day treatment period (D) are shown. n = 7 KO animals and 8 Re^L animals. Data in shown B mean ± SEM; different letters signify conditions that are statistically different (P < 0.05) by ANOVA with Tukey’s post hoc test. For C and D, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 by ANOVA with Tukey’s post hoc test.