TABLE 2.

Effects of AmB resistance on the fluorescence of exponential-phase L. donovani promastigotes labeled with Nile red and on the membrane fluidity of promastigotes as assessed by emission anisotropy of TMA-DPH^a

Promastigote characteristic	IRFb	IYFc	IYF/IRF	rd
Amb sensitive	50.2 ± 1.2	19.3 ± 0.5	92.8 ± 0.6	0.152 ± 0.001
Amb resistant	48.4 ± 0.9	15.6 ± 0.1	77.4 ± 0.5	0.031 ± 0.001

^aCell staining and measurements were performed as described in Materials and Methods. Values are means ± standard deviations for three experiments on untreated L. donovani DD8 AmB-sensitive and AmB-resistant promastigotes. 

^bIRF, intensity of red fluorescence. 

^cIYF, intensity of yellow fluorescence. 

^dr, measure of fluorescence anisotropy of TMA-DPH.