Figure 2.

Preserved expression of engineered CD123 variants despite abolished binding to the mAb MIRG123. (A and B) Flow cytometry plots (A) and summarizing bar graph (B) showing binding of the anti-human CD123 antibody MIRG123 (biosimilar of CSL362) and the control clone 6H6 to wt CD123 and its 28 variants stably expressed in HEK-293 cells. Variants were categorized based on the dual staining to MIRG123 and 6H6 as non-binding (blue, <1% dual staining), weak (orange, 1–20%), or strong (red, >20%) binding variants. Control conditions (gray) are HEK-293 cells stably expressing wt CD123 (HEK-CD123) and non-transduced HEK-293 cells (HEK). Error bars: mean (SD). Data in A are representative of three independent experiments summarized in B.