Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Sep 29;222(12):e202301069. doi: 10.1083/jcb.202301069

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 Scott et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 4. — Inactive PLK4 stably binds to the parent centriole. (A) Immunofluorescence images of endogenously tagged PLK4-mNeonGreen (green), CEP152 (red), and CEP192 (white) in DLD1 cells treated with DMSO (top), centrinone (middle), or MLN4924 (bottom). (B) Quantification of the fold change in centriolar PLK4-mNeonGreen upon treatment with DMSO (black), centrinone (red), or MLN4924 (blue) for 4 h prior to fixation. Fold change was normalized to DMSO. Triangles mark individual cells and circles mark the average for each experiment. (C) FRAP analysis of PLK4-mNeonGreen in S-phase DLD1 cells displaying iRFP-PCNA puncta. Cells were treated with DMSO (black), centrinone (red), or MLN4924 (blue). n refers to the total number of cells analyzed. (D) Schematic of the reporters used to define the cell cycle stage in DLD1 cells expressing endogenously tagged PLK4-mNeonGreen. Drugs were added at least 1 h prior to FRAP. (E) FRAP analysis of PLK4-mNeonGreen in G1 (blue), S (purple), or G2 (red) phase treated with DMSO or centrinone. Recovery in centrinone was also measured. The S-phase FRAP is replotted from C. Drugs were added at least 1 h prior to FRAP. n refers to the total number of cells analyzed. Data are represented as mean ± SEM. Scale bar = 5 µm. Inset scale bar = 500 nm.