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. 2023 Aug 23;42(40):2956–2970. doi: 10.1038/s41388-023-02814-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A–C WB verified NCF2 knockdown efficiency in T24 and 5637 cells. D–I HUVEC tube formation and transwell migration assays were used to investigate the effects of NCF2 knockdown on pro-angiogenesis and migratory abilities of T24 cells (D–F) and 5637 cells (G–I). Scale bars: 2 mm (black lines), 500 μm (orange lines). J Fluorescence in situ hybridization (FISH) assay detected the subcellular distribution of BLACAT3 in T24 and 5637 cells. 18 S rRNA and U6 were respectively used as cytoplasmic and nuclear internal reference biomarkers. Scale bars: 20 μm. K, L The subcellular distribution of BLACAT3 in T24 and 5637 cells was detected by qRT-PCR assay after isolation of nuclear and cytoplasmic RNAs. GAPDH and U6 were respectively used as cytoplasmic and nuclear internal reference biomarkers. Statistical significance was assessed using two-tailed Student’s t test between two groups, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.