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Subcellular localization of AXL in U251-MG cells U251-MG cells were grown on glass coverslips, fixed, and permeabilized with 0.2% (v/v) Triton X-100. After immunostaining with anti-AXL antibody, the coverslips were mounted on Vectashield and examined using a Zeiss confocal microscope. Scale bars: 10 μm
