Fig. 4.

AXL regulates the stabilization and nuclear translocation of HIF-1α. a Immunoblot analysis with the indicated antibodies of lysates from U251-MG cells that were transfected with control siRNA or AXL siRNA under CoCl₂ treatment. b U251-MG cells were grown on glass coverslips and transfected with control siRNA or AXL siRNA. Next, the cells were treated with or without 150 μM CoCl₂ for 24 h, fixed, and permeabilized with 0.2% (v/v) Triton X-100. After immunostaining with anti-HIF1 antibody, the coverslips were mounted on Vectashield and examined using a Zeiss confocal microscope. Scale bars: 20 μm. c U251-MG cells were grown on glass coverslips and treated with or without 150 μM CoCl₂ for 24 h. The cells were then fixed and permeabilized with 0.2% (v/v) Triton X-100. After immunostaining with anti-AXL antibody, the coverslips were mounted on Vectashield and examined using a Zeiss confocal microscope. Scale bars: 20 μm