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. 2023 Sep 29;8:143. doi: 10.1038/s41541-023-00729-4

Fig. 2. RSV F-specific CD4+ T cell responses in mice.

Fig. 2

Naive CB6F1 mice received three injections, two weeks apart (Days 0, 14, 28). They were administered AS01-adjuvanted RSVPreF3 vaccine formulated with high-dose (HD), medium-dose, (MD) or low-dose (LD) AS01 (n = 13, 13 or 9/timepoint, respectively), non-adjuvanted RSVPreF3 vaccine (Non-Adj.; n = 9/timepoint), or saline only (controls; n = 6/timepoint). Intracellular cytokine staining was performed on splenocytes collected 14 days after the second and third doses (Days 28 and 42, respectively), after restimulation with peptide pools covering the RSVPreF3 sequence. Frequencies of RSV F-specific CD4+ T cells expressing at least two markers among IL-2, IFN-γ and TNF-α are presented as geometric means with 95% confidence intervals (bars) and as individual frequencies (symbols). **P < 0.01. ***P < 0.001 (ANOVA for repeated measures). For geometric mean calculations, values of 0% were assigned a value of 0.005% (i.e., half of the minimum detected value), as indicated by the horizontal dotted line.