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. 2023 Sep 30;14(9):644. doi: 10.1038/s41419-023-06165-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A Protocol based on tissue from SEE-FIM protocol. After IHC against p53 and Ki-67, the coverslip glass and the mounting medium are removed to access the tissue section. Digestion of the lesion is performed directly on the tissue section and a droplet-based liquid extraction is performed to recover the peptides before MS-based proteomics analysis. B Pre-neoplastic lesions found in the fallopian tube are defined by p53 positivity and Ki-67 index. For p53 signature: accumulation of p53 in at least 12 cells without morphological abnormalities and low Ki-67 index; STIL: same accumulation of p53 in more than 20 cells with some morphological abnormalities and a higher Ki-67 proliferating index (10–40%); STIC: high p53 and Ki-67 index and cells atypical morphology (carcinoma-like).