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. 2023 Jan 7;122(18):3630–3645. doi: 10.1016/j.bpj.2023.01.005

Figure 4.

Figure 4

Laser ablation to disrupt mechanical homeostasis of acini. (A) Schematic of the laser ablation experiment; green color depicts podocalyxin. (B) Time-lapse DIC and fluorescent GFP-podocalyxin images (GFP) of a 7-day-old acinus that was ablated with the laser in the red box (see Video 13); red arrow depicts damaged area from which fluid flows out. Corresponding outlines show podocalyxin-labeled surface (green) and acinar outer contour (black). Scale bar, 20 μm. (C) Time-lapse fluorescent GFP-podocalyxin images (GFP) of a 7-day-old control acinus post ablation (see Video 14). Scale bar, 20 μm. Corresponding outlines show podocalyxin-labeled surface (green) and acinar outer contour (black). Images in (C) are representative of six out of eight acini.