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. 2023 Aug 1;122(18):3768–3782. doi: 10.1016/j.bpj.2023.07.030

Figure 1.

Figure 1

Experimental workflow imaging mitochondrial fission by cryo-ET. (1) Mammalian cells were grown on fibronectin-coated EM grids for 24–48 h and treated with FCCP for 3–6 min to induce mitochondrial fission. (2) Samples were then plunge frozen and (3) cryo-FIB milled to create thin lamellae randomly positioned near the middle of the cell. (4) Lamellae were imaged by cryo-ET to create tilt series, sets of projection images obtained by iteratively tilting the sample that are used to (5) computationally reconstruct a 3D volume (a tomogram). Images in (3) are adapted from (22).