Figure 1.

Fibroblasts extend flat, roughly uniform, and steady-state protrusions over PLL-g-PEG from surface-patterned Fn attachment sites. (A) Schematic of cell-plating conditions for the analysis of actin dynamics in steady-state “spillover” protrusions over PLL-g-PEG-coated glass surfaces from surface patterned Fn attachment sites. (B) GFP-actin stably expressed in JR20 cells (mouse dermal fibroblasts) that has been plated as schematized in (A) was visualized and captured in a series of images along the Z-plane, displayed as a maximum intensity projection. A cross section from the Z-stack projected in (B) is shown in (b’), and a pseudo side view of a 3D reconstruction is shown in (b”). Scale bars, 10 μm. (C) Graph of the approximate average cell protrusion distance outward from the edge of micropatterned Fn shapes in JR20s stably expressing GFP-actin. Bars represent the mean ± standard deviation (n = 56 protrusions from 3 independent experiments). To see this figure in color, go online.