Figure 3.

Experimental inputs that constrain parameterization of quantitative models. (A) For a cohort of seven cells, the full span of the nonadhesive region was rapidly bleached. For each cell, the span of region L was estimated from the prebleach image (red dashed lines). The spatial profiles of GFP-actin fluorescence pre- and postbleach were averaged across most of the width of the bleached area (yellow dashed rectangle). F-Actin retrograde flow velocity, V_F, was estimated from the associated kymograph. Scale bar, 3 μm. (B) Plot of the spatial profiles of GFP-actin fluorescence pre- and postbleach. At a suitable distance from the leading edge, x = x_match, the normalized, prebleach fluorescence was attributed to the sum of F- and G-actin [f(x_match) + g(x_match)], whereas the normalized fluorescence ∼5 s postbleach was attributed to G-actin [g(x_match)]. (C) The four experimental estimates for each cell in the cohort (each with a unique symbol) are plotted. To see this figure in color, go online.