FIG. 3.

Results of PCR for E. canis detection demonstrating enhanced sensitivity of the seminested method. (A) By using first-step primers, a 780-bp PCR product can be visualized in lane 1 (positive control, cultured E. canis) but not in lanes 2 to 11 (DNA extracted from EDTA-anticoagulated blood samples from dogs 2 to 11, respectively). (B) A seminested PCR product yielding a 220-bp band can be identified for the positive control (lane 1) and blood samples from dogs 2, 4, 6, 8, 9, and 10 (the same samples tested in panel A). Lane M, 100-bp DNA ladder (Promega). Numbers on the left are in base pairs.