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. 2023 Mar 13;10(5):uhad044. doi: 10.1093/hr/uhad044

Figure 5.

Figure 5

The expression levels of PsSKP1 during chilling- and GA3-induced bud dormancy release, and negative regulation of chilling-induced dormancy release in tree peony buds by interaction between PsF-box1, PsSKP1, and PsRGL1. A and B Expression of PsSKP1 after chilling and exogenous GA3 treatment by qRT-PCR. C Interaction between PsF-box1 and PsSKP1 by yeast two-hybrid assay in QDO (SD/−Leu/−Trp/-His/−Ade) medium with AbA of 200 ngInline graphic mL−1 and X-α-gal of 20 mg.mL−1. D Interaction between PsF-box1 and PsSKP1 by pull-down assay. Data represent the mean ± SD of six replicates. *P < 0.05; **P < 0.01.