FIG. 5.

l-FMAU inhibits DHBV DNA synthesis in DHBV DNA-infected primary duck hepatocytes. l-FMAU or β-l-F-ddC was added at the indicated concentrations 3 days after plating for 6 days. Cells were harvested at the end of therapy (day 10 [D10]) and 3 days after drug release (day 13 [D13]). The viral DNA released in the supernatant of primary hepatocyte culture was analyzed by a dot blot assay (A). Intracellular protein-bound (B) and protein-free (C) viral DNAs were extracted and subjected to Southern blot analysis. The positions of relaxed circular (RC), linear (L), CCC, and single-stranded (SS) DNAs are indicated. The results of a typical experiment are shown.