[Preprint]. 2023 Oct 5:2023.09.18.558367. Originally published 2023 Sep 22. [Version 2] doi: 10.1101/2023.09.18.558367

Table 1.

Activity of designed variants using generative AI

Enzyme	Generative Model	Mutant Type	Functional Assay Comment	Activity Assay Comment
Luciferase RLuc	MaxEnt (this study)	Single		4 out of 8 designs show enhanced activity compared to wild-type; maximum fold increase: 2.0
Chorismate Mutase	MaxEnt ¹⁵	Multiple	481 out of 1618 are functional	5 tested designs exhibit comparable activity to natural enzymes
Malate Dehydrogenase	GAN ¹⁶	Multiple	13 out of 55 are functional
Luciferase LuxA	VAE ¹⁷	Multiple	9 out of 11 are functional
Lysozyme	Language ¹⁸	Multiple		6 tested designs exhibit comparable activity compared to wild-type
Ornithine Transcarbamylase	VAE ¹⁹	Multiple		58 out of 87 designs show enhanced activity compared to wild-type; maximum fold increase: 2.5

GAN: Generative Adversarial Network; VAE: Variational Autoencoder; Language: Language Model