(A) Feeding rate of L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 30°C at CT13. (B–D) Sleep duration (B), bout number (C), and bout length (D) of L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 30°C. (E–G) Arousal threshold (E), long-term aversive memory performance (F), and short-term aversive memory performance (G) in L3 expressing npf-Gal4>UAS-TrpA1 and genetic controls at 30°C. (H,I) Short-and long-term term aversive memory performance in L3 raised on ctrl and L.S. food. A, n=18–20 larvae; B-D, n=24–61 larvae; E, n=125–160 sleep episodes, 18 larvae per genotype; F–I, n=8 PIs (240 larvae) per genotype. One-way ANOVAs followed by Sidak’s multiple comparisons tests [(A) and (E–G)]; Two-way ANOVAs followed by Sidak’s multiple comparison test [(B-D)]; unpaired two-tailed Student’s t-test [(H) and (I)].