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[Preprint]. 2024 May 22:2023.09.19.558472. Originally published 2023 Sep 22. [Version 3] doi: 10.1101/2023.09.19.558472

Figure 3: Deeper sleep in L2 is energetically disadvantageous.

Figure 3:

(A, B) Sleep duration (A) and arousal threshold (B) of L2 control fed vehicle control (L2) or Gaboxadol (L2 Gab). (C, D) Total body weight of L2 (C) (in groups of 10) or L3 (D) (in groups of 5) fed vehicle control or Gaboxadol (Gab). (E) Long-term aversive memory performance in L2 fed vehicle control (L2) or Gaboxadol (L2 Gab). (F, G) Sleep duration (F) and arousal threshold (G) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. (H) Long-term aversive memory performance of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. (I, J) Total body weight (I) and total body length (J) of L2 expressing R76G11-Gal4>UAS-TrpA1 and genetic controls at 30°C. A, n=28 larvae; B, n=110–220 sleep episodes, 18 larvae per genotype; C, n=5–7 groups (50–70 larvae); D, n=8 groups (40 larvae); E, n=8 PIs (240 larvae) per genotype; F, n=33–36 larvae; G, n=234–404 sleep episodes, 30–40 larvae per genotype; H, n=8 PIs (240 larvae) per genotype; I, n=5 groups (25 larvae); J, n=31–32 larvae. Unpaired two-tailed Student’s t-tests [(A-E)]; one-way ANOVAs followed by Sidak’s multiple comparisons tests [(F-J)].