Figure 7. Systemic CFTR potentiation rescues AWL secretion and blocks alveolar SA^GFP stabilization in IAV-infected mice.

Group data quantify confocal images of live, intact, perfused lungs. Mice were given intranasal instillation of IAV, then, at 6 hours, intraperitoneal injection of vehicle (Veh) or ivacaftor (Ivac) as indicated. (A) Lungs were excised for imaging at 24 hours after IAV instillation, and alveoli were microinstilled with TRITC-labeled dextran. Data show change of dextran fluorescence in alveolar airspaces. (B) At 24 hours after IAV instillation, mice were intranasally instilled with SA^GFP, then the lungs were immediately excised for imaging. Data show spontaneous change of SA^GFP microaggregate (MA) fluorescence in alveolar airspaces from 1–3 hours after intranasal SA^GFP instillation. Circles indicate n and each represent 1 mouse in which change of dextran (A) or SA^GFP (B) fluorescence was quantified in imaging fields of at least 30 alveoli. Bars represent mean ± SEM; *P < 0.05 by 2-tailed t test.