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. 2023 Sep 30;21:355. doi: 10.1186/s12951-023-02129-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — BT-EXO-CAP induced ferroptosis in human OS cells. (A) Representative TEM picture of mitochondria. (B) Flow cytometry-based detection of FerroOrange fluorescence in OS cells. (C) DCFH-DA fluorescence of OS cells captured by fluorescence microscope. (D) Flow cytometry-based detection of C11-BODIPY fluorescence in OS cells. (E) Measurement of ferroptosis-related protein expressions via WB. (F) GSH levels in OS cells. n = 6. (G) Cell viability of OS cells. n = 10. (H) Flow cytometry-based detection of FerroOrange and (I) C11-BODIPY fluorescence in OS cells. (J) GSH levels in OS cells. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001. Scale bar: A 1 μm, C 100 μm