Figure 4.

IHC for TGF β-1 and collagen type IV in corneas at six months after irregular PTK and treatment with topical BSS vehicle or topical losartan six times per day. (A) Representative IHC images from the center of each cornea. TGF-β1 was variably localized in the apical epithelium and consistently throughout the anterior stroma in each cornea of both groups. Collagen type IV (green) was deposited at high levels in the anterior stroma in all corneas in both groups. e is epithelium. Asterisk indicates artifactual dislocations of epithelium from stroma that occur during sectioning. The yellow rectangles are 200-pixel × 75-pixel ImageJ quantitation areas. (B) Although the mean anterior stromal TGF-β1 trended higher in losartan-treated corneas, there was no statistically significant difference, whether or not the cornea that developed an epithelial defect (LOS no. 2) was included in the analysis.