Table.
Antibodies Used in Immunohistochemistry
| Antigen | Source | Species Source | Ab Isotype | Catalog Number | Dilution |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| α-SMA | DAKO | Mouse | IgG2a | M0851 | 1:400 |
| Vimentin | Abcam | Chicken | IgY | ab24525 | 1:2000 |
| Keratocan | Winston Kao, Ph.D. | Goat | IgG | — | 1:200 |
| TGF-β1 | GeneTex | Mouse | IgG1 | GTX21279 | 1:100 |
| Collagen type IV | EDM Millipore | Goat | IgG1 | AB769 | 1:2000 |
| Secondary antibodies | |||||
| Alexa Fluor 568 anti-mouse | Thermo Fisher Scientific | Donkey | IgG | A10037 | 1:200 |
| Alexa Fluor 488 anti-goat | Thermo Fisher Scientific | Donkey | IgG | A11055 | 1:200 |
| Alexa Fluor 488 anti-mouse | Thermo Fisher Scientific | Donkey | IgG | A21202 | 1:200 |
| Alexa Fluor 568 anti-goat | Thermo Fisher Scientific | Donkey | IgG | A11057 | 1:200 |
| Alexa Fluor 647 anti-chicken | Jackson ImmunoResearch Inc. | Donkey | IgY | 703-605-155 | 1:400 |