Figure S3.

Myeloid and T cell distribution and activation profile are not significantly different in mice with B cell–specific Myd88 deletion after disease onset. The T cell compartment was analyzed for 20-wk-old male Tam-hCD20-Cre;-Myd88^fl MRL.Fas^lpr (TamCreB ΔMyd88) (n = 12–13) or Cre negative (Ctrl) (n = 16) MRL.Fas^lpr mice after 8 wk of tamoxifen treatment. (A–C) Distribution of TCRβ gated population, expressing CD4, CD8, or neither (DN T cells) as a percent of live cells (B) CD4 T cell distributions: naïve (CD62L⁺, CD44⁻), central memory T cells (T_CM) (CD62L⁺, CD44⁺), effector memory T cells (T_EM) (CD62L⁻, CD44⁺) as a % of TCRβ+CD4⁺ T cells, with representative FACS plot in the right panel and (C) CD8 T cells distributions: naïve (CD62L⁺, CD44⁻), T_CM (CD62L⁺, CD44⁺), T_EM (CD62L⁻, CD44⁺) as a % of TCRβ+CD8⁺ T cells with representative FACS plot in the right panel. (D) Distribution of CD11c⁺, MHCII⁺, CD19⁻ cDCs as a percent of live cells with representative FACS plot in the left panel. (E) Distribution of CD317⁺, SiglecH⁺, CD19⁻ pDCs as a percent of live cells with representative FACS plot in the left panel. (F) Distribution of CD11b⁺CD19⁻ myeloid cells. (G) Distribution of macrophages CD11b⁺, F4/80⁺, Ly6g/c⁻ macrophages, CD11b⁺, F4/80^int, Ly6g/c^int monocytes, and CD11b⁺, F4/80⁻, Ly6g/c⁺ neutrophils as a percent of live cells with representative FACS plot in the left panel. Scatter plots display data from individual mice with horizontal lines showing median values. P values as indicated, using two-tailed Mann–Whitney U test. Data shown are representative of two independent cohorts with data combined from all cohorts and n indicated for each experiment as shown.