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. 2023 Sep 29;14:6088. doi: 10.1038/s41467-023-41753-1

Fig. 2. Premature centriole disengagement during mild replication stress depends on the ATR/Chk1 axis of the DDR Pathway.

Fig. 2

A U-ExM images of centrioles in G2 Phase RPE1 cells, treated with indicated drugs/inhibitors targeting the DNA damage repair pathway. B Quantification of the percentage of G2 phase cells with engaged centrioles in their centrosomes (N = 3 independent experiments, n = 96, 93, 70, 71, 76 and 92 cells in DMSO, Aph, ATMi, ATRi, Aph+ATMi and Aph+ATRi, respectively; p-values from two-tailed Sídak test). C U-ExM images of centrioles in G2 Phase RPE1 cells, treated with indicated drugs/inhibitors targeting Chk1 or Chk2. D Quantification of the percentage of G2 phase cells with engaged centrioles in their centrosomes (N = 3 independent experiments, n = 95, 95, 92, 90 and 94 cells in DMSO, Aph, Aph+Chk1i-1, Aph+Chk1i-2 and Aph+Chk2i, respectively). Data presented as mean values ± SD. (p-values from two-tailed Sídak test). Scale bars = 0.5 µm. Source data for all graphs are provided as a Source Data file.