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. 2023 Sep 29;14:6088. doi: 10.1038/s41467-023-41753-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Live-cell time-lapse images of RPE1: EB3-eGFP/H2B-mCherry cells treated with indicated drugs/inhibitors. EB3-GFP channel is shown as a large image and the corresponding H2B-mCherry (DNA) channel is shown as insets. Scale bars = 5 µm. B Quantification for the proportion of mitotic cells displaying transient multipolar spindles upon mitotic entry in (A) (N = 3 independent experiments, n = 485, 137, 462, 385, 333 and 276 cells in DMSO, Aph, Wee1i-1, Wee1i-2, Aph+Wee1i-1 and Aph+wee1i-2, respectively: p-values from two-tailed Sídak test). C Schematic representation of auxin-induced cyclin degradation of Cyclin-A2 and B1 in RPE1 cells. D Expansion microscopy images of centrioles in G2 Phase RPE1 OSTIR1, Cyclin-A2 double degron (CCNA2-DD) and Cyclin-B1 double degron (CCNB2-DD) cells after inducing degron expression and treated with indicated drugs/inhibitors. E Quantification of percentage of G2 phase cells with disengaged centrioles in their centrosomes [N = 3 independent experiments, n = 88 (OSTIR:DMSO), 86 (OSTIR:Aph), 85 (OSTIR:Aph+Cdk1i), 88 (CCNA2-DD:DMSO), 86 (CCNA2-DD:Aph), 86 (CCNA2-DD:Aph+Cdk1i), 85 (CCNB1-DD:DMSO), 75 (CCNB1-DD:Aph) and 70 (CCNB1-DD:Aph+Cdk1i) cells]. Data presented as mean values ± SD. Data presented as mean values ± SD. (p-values from two-way ANOVA and Dunn’s multiple comparison test). Scale bars = 0.5 µm. Source data for all graphs are provided as a Source Data file.