Fig 4.

BP4L-18:1:1 is excluded from the CNS and shows variable access to peripheral tissues. (a, b) Following once-daily dosing by gavage for 7 days, blood and tissue samples were taken from 12 randomly selected animals (three animals from each of the four plus-BP4L-18:1:1 behavioural cohorts reported in Fig. 5). The box plots in (a) report the BP4L-18:1:1 accumulation in each tissue relative to the blood concentration in the same animal. Note that tissue loads of pmol mg⁻¹ wet weight are equivalent to μM, assuming the tissue density is 1^61,62 and the drug can distribute freely across the entirety of the tissue. Based on the overlap in the ratios of the high and low dose and male and female populations (as per (a)) and a lack of difference between the tissue loads of male vs female (P=0.464) and high dose vs low dose (P=0.430) (as per Student's t-test comparisons), (b) reports the mean tissue loads [standard deviation] across the combined samples (males plus females at both high and low doses). (c) A Friedman repeated measures analysis of variance on the tissue loads indicates brain, dorsal root ganglia (DRG), and kidney are protected organs relative to blood, whereas lung, heart, and liver are in near equilibrium with blood. For full statistical comparisons, see Supplementary Table S5.