Table 2.

Effect of single/two-step antioxidant extraction from ghee on the time of color change of DPPH solution

Concentration of antioxidant added in ghee (%)	Single step extraction		Two- step extraction
	BHA (600 µL)	TBHQ (600 µL)	BHA (~ 2000µL)	TBHQ (~ 2000µL)
	Time (min)		Time (min)
0	86 ± 4.00E	82 ± 3.00E	240 ± 7E	243 ± 9E
0.02	1.00 ± 0.25A	1.00 ± 0.25A	3.00 ± 0.25A	1.00A
0.01	1.83 ± 0.29B	1.00 ± 0.29A	6.00 ± 0.50B	2.00 ± 0.50B
0.005	2.50 ± 0.5C	1.00 ± 0.50A	12.66 ± 1.00C	3.00 ± 0.50C
0.0025	3.00 ± 0.50D	1.83 ± 0.50B	27.00 ± 1.50D	6.00 ± 0.50D

Values are represented as Mean ± SD, n = 3; ^A−E–different superscript column-wise differ significantly (p < 0.05)