. 2023 Aug 16;66(11):2117–2138. doi: 10.1007/s00125-023-05981-w

Table 2.

Body weight and tissue weights in C57BL/6J mice under normal and experimental conditions

Tissue	Control	HFS	pA1c	PC	BGC	pA1c+PC	pA1c+BGC	pA1c+PC+BGC	ANOVA (p value)
Body weight (BW) (g)	25.47±0.78^a	37.26±1.18^b	37.63±1.12^b	38.82±1.23^b	38.52±1.07^b	36.75±1.34^b	38.18±1.40^b	38.69±1.19^b	<0.001
Tissue weight (prop. total BW)
Liver	1.05±0.10	1.12±0.05	1.18±0.05	1.21±0.05	1.23±0.09	1.18±0.09	1.13±0.06	1.17±0.05	NS
Spleen	0.07±0.01	0.09±0.01	0.08±0.01	0.09±0.01	0.08±0.01	0.08±0.01	0.08±0.01	0.09±0.01	NS
Kidney	0.17±0.01	0.18±0.01	0.18±0.01	0.18±0.01	0.18±0.01	0.18±0.01	0.18±0.01	0.18±0.01	NS
Gastrocnemius muscle	0.15±0.01^a	0.16±0.01^a	0.17±0.01^a	0.17±0.01^a	0.17±0.0^a	0.17±0.01^a	0.17±0.01^a	0.19±0.01^b	<0.01
WAT depot weight (prop. total BW)
Mesenteric fat	0.54±0.09^a	2.05±0.13^b	1.41±0.17^c	1.80±0.16^b	1.67±0.15^b	1.19±0.17^c	1.58±0.13^b	1.33±0.12^c	<0.001
Epididymal fat	1.74±0.29^a	6.05±0.17^b	5.17±0.53^b	5.37±0.25^b	5.48±0.30^b	5.14±0.29^b	5.21±0.31^b	4.36±0.31^c	<0.001
Subcutaneous fat	0.41±0.08^a	1.90±0.22^b	1.58±0.25^b	1.81±0.19^b	1.82±0.15^b	1.71±0.22^b	1.64±0.24^b	1.39±0.10^b	<0.001
Retroperitoneal fat	0.35±0.07^a	1.43±0.10^b	1.32±0.15^b	1.53±0.10^b	1.57±0.12^b	1.57±0.13^b	1.42±0.12^b	1.25±0.11^b	<0.001
Visceral WAT^d	2.63±0.34^a	9.07±0.32^b	7.90±0.58^b	8.70±0.30^b	8.71±0.41^b	7.90±0.48^b	8.21±0.38^b	7.23±0.37^c	<0.001
Total WAT^e	3.04±0.41^a	10.97±0.43^b	9.49±0.79^b	10.51±0.39^b	10.53±0.47^b	9.61±0.57^b	9.85±0.52^b	8.51±0.39^c	<0.001
BAT depot weight (prop. total BW)
Brown fat	0.25±0.02^a	0.40±0.01^b	0.44±0.07^b	0.46±0.03^b	0.52±0.03^b	0.47±0.03^b	0.50±0.04^b	0.58±0.06^c	<0.001

Data are expressed as mean ± SEM

^aStatistically equal to the control group

^bStatistically equal to the HFS group

^cStatistically different from the control and HFS groups

^dVisceral WAT, sum of mesenteric, epididymal and retroperitoneal fat depots

^eTotal WAT, sum of visceral WAT and subcutaneous fat

Statistical analyses were performed using the one-way ANOVA test followed by the SNK multiple comparisons test when statistical significance (p<0.05) was reached in the ANOVA test between the eight groups

prop., proportion