Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1987 Sep;85(1):204–211. doi: 10.1104/pp.85.1.204

Membrane Deterioration in Senescing Carnation Flowers 1

Coordinated Effects of Phospholipid Degradation and the Action of Membranous Lipoxygenase

Maribeth Fobel 1, Daniel V Lynch 1,2, John E Thompson 1
PMCID: PMC1054230  PMID: 16665659

Abstract

The lipid fluidity of microsomal membranes from the petals of cut carnation flowers decreases as the flowers senesce. A comparable change in fluidity was induced by in vitro aging of microsomal membranes from young flowers under conditions in which membranous lipoxygenase-like activity was active. There was no change in fluidity when the membranes were aged in the presence of inhibitors of lipoxygenase or were heat-denatured prior to aging. Membranes from naturally senesced flowers and membranes that had been aged in vitro both sustained an increase in saturated:unsaturated fatty acid ratio that accounted for the decrease in lipid fluidity, and in both instances there was evidence for depletion of the unsaturated fatty acids, linoleic acid, and linolenic acid, which are substrates for lipoxygenase. Loss of lipid phosphate reflecting breakdown of membrane phospholipids preceded the depletion of unsaturated fatty acids attributable to the lipoxygenase-like activity. The data have been interpreted as indicating that fatty acid substrates for membrane-associated lipoxygenase-like activity are made available by the initiation of phospholipid degradation, and that the utilization of these substrates results in a selective depletion of unsaturated fatty acids from the membrane and an ensuing decrease in bulk lipid fluidity.

Full text

PDF
204

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  2. Beutelmann P., Kende H. Membrane Lipids in Senescing Flower Tissue of Ipomoea tricolor. Plant Physiol. 1977 May;59(5):888–893. doi: 10.1104/pp.59.5.888. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  4. Chia L. S., Thompson J. E., Dumbroff E. B. Simulation of the effects of leaf senescence on membranes by treatment with paraquat. Plant Physiol. 1981 Mar;67(3):415–420. doi: 10.1104/pp.67.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hamilton J. G., Comai K. Separation of neutral lipids and free fatty acids by high-performance liquid chromatography using low wavelength ultraviolet detection. J Lipid Res. 1984 Oct;25(10):1142–1148. [PubMed] [Google Scholar]
  6. Itaya K., Ui M. A new micromethod for the colorimetric determination of inorganic phosphate. Clin Chim Acta. 1966 Sep;14(3):361–366. doi: 10.1016/0009-8981(66)90114-8. [DOI] [PubMed] [Google Scholar]
  7. Kanofsky J. R., Axelrod B. Singlet oxygen production by soybean lipoxygenase isozymes. J Biol Chem. 1986 Jan 25;261(3):1099–1104. [PubMed] [Google Scholar]
  8. MORRISON W. R., SMITH L. M. PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964 Oct;5:600–608. [PubMed] [Google Scholar]
  9. McKersie B. D., Lepock J. R., Kruuv J., Thompson J. E. The effects of cotyledon senescence on the composition and physical properties of membrane lipid. Biochim Biophys Acta. 1978 Apr 4;508(2):197–212. doi: 10.1016/0005-2736(78)90325-5. [DOI] [PubMed] [Google Scholar]
  10. Paliyath G., Thompson J. E. Calcium- and calmodulin-regulated breakdown of phospholipid by microsomal membranes from bean cotyledons. Plant Physiol. 1987 Jan;83(1):63–68. doi: 10.1104/pp.83.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pauls K. P., Thompson J. E. Evidence for the accumulation of peroxidized lipids in membranes of senescing cotyledons. Plant Physiol. 1984 Aug;75(4):1152–1157. doi: 10.1104/pp.75.4.1152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peterman T. K., Siedow J. N. Behavior of Lipoxygenase during Establishment, Senescence, and Rejuvenation of Soybean Cotyledons. Plant Physiol. 1985 Aug;78(4):690–695. doi: 10.1104/pp.78.4.690. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Thompson J. E., Mayak S., Shinitzky M., Halevy A. H. Acceleration of membrane senescence in cut carnation flowers by treatment with ethylene. Plant Physiol. 1982 Apr;69(4):859–863. doi: 10.1104/pp.69.4.859. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES