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. 2023 Sep 30;14:6119. doi: 10.1038/s41467-023-41860-z

Fig. 4. PAX4 p.Arg192His and p.Tyr186X donor-derived hiPSCs have perturbations in differentiation towards SC-islets.

Fig. 4

a Donor-derived hiPSC lines were generated from the following genotypes: three lines from two wildtype donors; four lines from two p.Arg192His donors; five lines from two p.His192His donors; and three lines from one p.Tyr186X donor. b–e Transcript expression of (b) PAX4, (c) INS, (d) GCG and (e) SST in hiPSC-derived endocrine progenitor (EP) cells using differentiation Protocol B. f–i Transcript expression of (f) PAX4, (g) INS, (h) GCG and (i) SST in hiPSC-derived islet-like cells (SC-islets) using differentiation Protocol B. j Representative immunofluorescence images of hiPSC-derived islet-like cells with C-peptide in red, glucagon in green, and nuclei in blue. Arrows indicate C-PEP + /GCG+ double-positive cells. Scale bar: 50 µm. k–m Quantification of immunofluorescence images for the percentage of cells expressing (k) GCG (monohormonal), (l) C-PEP (monohormonal) or (m) C-PEP + /GCG+ (polyhormonal). n Total insulin content normalized to total DNA from SC-islets. Data are presented as mean ± SEM. n = 4 independent differentiation experiments were performed in (b–i). n = 3 independent differentiation experiments were performed in (k–n). Statistical analyses were performed using one-way ANOVA. *p < 0.05, ***p < 0.001, and ****p < 0.0001. Differentiation protocol B was used to derive data in Fig. 4. Source data is provided in the Source Data File.