Table 4. D- and L-Amino acid contents in the culture supernatant and the bacteria cells of lactic acid bacteria (OPA-NAC derivatives).

	L.brevis IYO2091		L. plantarum IYO2092		L. plantarum IYO2179
	Culture supernatant	Bacterial cell	Culture supernatant	Bacterial cell	Culture supernatant	Bacterial cell
	μM	nmol/g1)	μM	nmol/g1)	μM	nmol/g1)
L-Asp	1,553	6,061	2,542	3,241	588	596
D-Asp	217	5,063	79	0	41	36
d/d+l ratio	0	0	0	0	0	0
L-Ser	2,358	700	76	0	17	49
D-Ser	28	644	29	0	0	0
d/d+l ratio	0	0	0	0	0	0
L-Gln	48	119	43	93	66	0
D-Gln	0	0	0	0	0	0
L-His	689	585	1,119	129	546	211
D-His	0	0	0	0	0	0
L-Arg	0	164	3,835	270	1,931	429
D-Arg	0	0	0	220	0	223
d/d+l ratio	0	0	0	0	0	0
L-Ala	2,787	2,808	3,937	807	1,834	213
D-Ala	1,065	2,884	1,273	843	596	362
d/d+l ratio	0	1	0	1	0	1
D-allo-Thr	0	0	152	0	0	97
L-Tyr	682	89	950	0	439	0
D-Tyr	0	0	0	0	0	0
L-Val	3,078	799	5,446	174	2,834	196
D-Val	0	0	0	0	0	0
L-Met	1,130	0	1,810	0	861	0
D-Met	0	0	0	0	0	0
L-Trp	0	202	1,379	0	663	0
D-Trp	0	0	0	0	0	0
L-Phe	2,446	365	4,085	94	2,054	74
D-Phe	0	0	0	0	0	0
L-Ile	2,460	494	4,072	101	2,091	129
D-allo-Ile	0	0	0	0	0	0
L-Leu	5,673	1,050	9,684	308	5,332	319
D-Leu	0	0	0	0	0	0

¹⁾Amount of substance (nmol) per 1 g of wet weight of cells calculated from concentration (μM).

OPA: o-phthaldialdehyde; NBC: N-tert-butyloxycarbonyl-L-cysteine.