Table 5. D- and L-Amino acid contents in the culture supernatant and the bacteria cells of lactic acid bacteria (OPA-NBC derivatives).

	L.brevis IYO2091		L. plantarum IYO2092		L. plantarum IYO2179
	Culture supernatant	Bacterial cell	Culture supernatant	Bacterial cell	Culture supernatant	Bacterial cell
	μM	nmol/g1)	μM	nmol/g1)	μM	nmol/g1)
L-Asp	1,419	5,227	1,069	2,855	553	526
D-Asp	162	3,985	0	0	21	31
d/d+l ratio	0	0	0	0	0	0
L-Glu	2,447	12,958	3,357	2,805	3,206	265
D-Glu	270	12,589	131	2,658	79	1,715
d/d+l ratio	0	0	0	0	0	1
L-Asn	1,002	0	689	1,425	661	1,329
D-Asn	0	0	0	0	0	0
L-Ser	2,517	652	0	111	0	75
D-Ser	0	610	0	0	0	0
d/d+l ratio	0	0	0	0	0	0
L-Gln	0	241	0	51	56	0
D-Gln	220	0	192	0	216	0
d/d+l ratio	1	0	1	0	1	0
L-His	613	454	470	111	445	200
D-Arg	0	0	0	211	0	218
L-Ala	2,891	2,744	2,070	840	1,838	228
D-Ala	1,004	2,636	573	748	540	317
d/d+l ratio	0	0	0	0	0	1
D-Tyr	0	0	0	0	0	0
L-Val	3,445	839	3,161	183	3,054	210
D-Val	0	0	0	0	0	0
L-Met	1,085	0	947	0	874	0
D-Met	0	0	0	0	0	0
L-Ile	2,719	487	2,277	104	2,198	136
L-Trp	673	0	621	0	597	0
L-Phe	2,505	341	2,142	83	2,032	67
D-Phe	0	0	0	0	0	0
L-Leu	5,132	951	4,843	293	4,708	297
D-Leu	0	0	0	0	0	0

¹⁾ Amount of substance (nmol) per 1 g of wet weight of cells calculated from concentration (μM).

OPA: o-phthaldialdehyde; NBC: N-tert-butyloxycarbonyl-L-cysteine.