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[Preprint]. 2023 Sep 22:2023.09.21.558268. [Version 1] doi: 10.1101/2023.09.21.558268

Figure 3. Impaired BMP signaling perturbs decidualization in the endometrium of individuals with endometriosis.

Figure 3.

A, B) Lysates from endometrial stromal cells of individuals without (A, “normal”) with endometriosis (B) after 2, 4, 6 or 8 days of EPC treatment were probed with antibodies to detect phosphorylated SMAD1/5 (pSMAD1/5), total SMAD1, total SMAD5, or GAPDH expression. C) Densitometric analysis of pSMAD1/5 in the EPC-treated stromal cells from individuals without (n=5) or with endometriosis (n=4). D,E) Quantitative reverse transcriptase PCR (qRT-PCR) was used to determine the expression of BMP2 and IGFBP1 in the endometrial stromal cells from individuals without (C, n=4) or with endometriosis (D, n=4). F,G) Time course analysis from the RNAseq analysis comparing the increasing gene expression patterns of ID1 and ID3 in normal and decreasing gene expression pattern in endometriosis stromal cells. Histograms (C,D,E) represent mean +/− standard error of the mean analyzed using a One Way ANOVA with Tukey’s posttest for multiple comparisons (C). EPC, 35nM estradiol, 1µM medroxyprogesterone acetate, 50µM cAMP.