Figure 5. UppS^UN demonstrates decreased enzymatic rate and results in decreased cellular Und-P than UppS^VU.

(A) UppS activity with 2CNA-GPP, a fluorescent analogue of the intrinsic substrate FPP. A. baumanni UppS from strains 17978VU (green) and 17978UN (blue) and were tested with the 2CNA-GPP substrate analogue and fluorescence increase upon elongation was monitored at an excitation wavelength of 340 nm and emission of 390 nm. Data are representative of 3 experiments. (B) Mass spectrometry quantitation of undecaprenyl-phosphate in ATCC 17978VU and 17978UN WT strains. Significance is by an unpaired t-test; **** p < 0.0001. (C) Silver stain of proteinase K-treated cell lysates to stain for LOS levels. Image is representative of 7 biological replicates. (D) Densitometric quantification of LOS bands as a ratio over 17978UN WT. n=6-7, data are means ± SEM, significance is by one-sample t-test compared to 1; * p < 0.05.