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. 2023 Mar 27;56(10):e13461. doi: 10.1111/cpr.13461

FIGURE 2.

FIGURE 2

The data of bioinformatics analysis and molecular biology experiment demonstrated that hBMSCs downregulated the expression of P2X3 in CFA‐rats. (A) Schematic overview of high‐throughput sequencing analysis workflow. (B) Heat map of the hierarchical clustering of 32 DEGs between CFA and CFA+ hBMSCs groups. (C) The volcano plot showed the upregulated DEGs (yellow) and downregulated DEGs (blue). (D) GO enrichment analysis revealed the different expressive proteins in BP, CC, and MF. (E) KEGG analysis predicted the probable pathway of DEGs. (F) The PPI network screened the two hub genes in DEGs. (G) The qRT–PCR result of P2X7. (H) Representative western blot image of P2X7 showed that it was no significant difference between CFA and CFA+ hBMSCs groups. GAPDH was the loading control. (I) The qRT–PCR result of P2X3 showed it was significantly decreased in the CFA+hBMSCs group. (J) Representative western blot image of P2X3. Tubulin was the loading control. *p < 0.05, **p < 0.01, ***p < 0.001. n = 3 each group.