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. 2023 Sep 30;13:16470. doi: 10.1038/s41598-023-43848-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proinflammatory cytokine levels were elevated in Ku80-deficient VSMCs. (A) mRNA levels of proinflammatory cytokines in cells were analyzed by real-time qPCR (n = 4). (B) Western blot analysis of ATM in Atm-silenced VSMCs. Both WT and Ku80^+/− cells were transfected with either negative control siRNA (siNC) or siRNA against Atm. Bands are cropped from the original blots presented in Supplemental Figure 9. (C) mRNA levels of IL-6 and MCP1 in Atm-silenced VSMCs were determined by real-time qPCR. *p < 0.05 compared with corresponding WT cells (n = 6). Statistical significance between groups was determined using the Mann–Whitney U test. Data are expressed as mean ± SEM.