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. 2023 Sep 30;14:6109. doi: 10.1038/s41467-023-41683-y

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© The Author(s) 2023, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Schematic representation of IFNγ inhibition experiment. Anti-IFNγ antibody (25 mg/kg) was injected intraperitoneally (i.p.) for two weeks (3 injections per week) in old mice. Mice were sacrificed for organ harvest 4 days after the last injection. Anti-IgG1 antibody were used as control. b qRT-PCR validation of Cd74 and H2-Ab1 gene expression levels in indicated conditions. n = 4 mice per group were analyzed. Error bars represent the SD. P value was calculated by two-sided Welch’s t test. c Representative pictures of anti-Olfm4 staining in indicated conditions. Scale bar, 20 µm. The dotted lines indicate the crypt structure. d–f The bar charts show the immunofluorescence quantification of the Olfm4⁺, Muc2⁺, and Chga⁺ cells in the indicated conditions. n = 4 mice per group were analyzed. Error bars represent the SD. P value was calculated by two-sided Welch’s t test. g, h The bar charts show the percentage of Ccl5⁺ CD4 T (cytotoxic) and Klrg1⁺ ILC2 cells quantified by FACS analysis in the indicated conditions. n = 4 mice per group were analyzed. Error bars represent the SD. P value was calculated by two-sided Welch’s t test. i Schematic representation of the in vivo IFNγ inhibition experiment for regeneration analysis. Anti-IFNγ antibody (25 mg/kg) was injected intraperitoneally (i.p.) every second day. 3 injections per week for 2 weeks were performed. One day after the last injection, 5-fluorouracil (5FU; 150 mg/kg) or DMSO as control were intraperitoneally (i.p.) injected. j Body weights of treated old animals shown as mean ± SD. n = 4 mice per group were analyzed. P value was calculated by two-sided Welch’s t test. k Representative picture of H&E-stained longitudinal sections of the proximal part of the intestine from indicated conditions. Scale bars 100 µm. l The bar chart shows the average number of crypts per millimeter of the proximal part of the small intestine of the indicated groups. n = 4 mice per group were analyzed. Error bars represent the SD. P value was calculated by two-sided Welch’s t test.