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. 2023;24(2):110–115. doi: 10.22099/IJVR.2023.43730.6410

Table 1.

The sequence of primers used for the detection of virulence factors by PCR reaction

Primer Primer sequence (5´→3´) Annealing Tm (°C) Size (bp) Reference
nucA F: 5´-GCGATTGATGGTGATACGGTT-3´ 53 276 Dehnad et al. (2020)
R: 5´-CAAGCCTTGACGAACTAAAGC-3´
mecA F: 5´-AGAAATGACTGAACGTCC-3´ 49 305 Dehnad et al. (2020)
R: 5´-ATTCCACATTGTTTCGGTC-3´
Tsst-1 F: 5´-ACAAGCGCTATTTTTATTTCAG-3´ 49 271 Zeinalpour Ahrabi et al. (2019)
R: 5´-CCCATCCCCAACCACTTTT-3´
Hla F: 5´-GTACAGTTGCAACTACCT-3´ 49 253 Dehnad et al. (2020)
R: 5´-CTTTCCAGCCTACTTTTTTATCAGT-3´
Hlb F: 5´-GTGCACTTACTGACAATAGTGC-3´ 49 313 Dehnad et al. (2020)
R: 5´-GTTGATGAGTAGCTACCTTCAGT-3´
Acme-arcA F: 5´-CTAGGTGCATAAATGTACGTG-3´ 49 577 Sabat et al. (2015)
R: 5´-CCAGAAGTACGCGAGAAC-3´
PVL F: 5´-AGGTAAAATGTCTGGACATG-3´ 49 427 Hoppe et al. (2018)
R: 5´-GCATCAACTGTATTGGATAGC-3´