TABLE 1.
Alterations in GyrA and ParC in clinical isolates of E. faecalis
| Strain | MIC (μg/ml)a
|
Amino acid (codon) at the positions indicatedb
|
|||||
|---|---|---|---|---|---|---|---|
| NFLX | OFLX | CPFX | GyrA
|
ParC
|
|||
| 83 | 87 | 80 | 84 | ||||
| Type strain | 6.25 | 3.13 | 1.56 | Ser (AGT) | Glu (GAA) | Ser (AGT) | Glu (GAA) |
| F26 | 6.25 | 1.56 | 1.56 | − | − | − | − |
| B21, B86 | 6.25 | 3.13 | 1.56 | − | − | Ser (AGC) | − |
| 138 | 6.25 | 3.13 | 1.56 | − | − | − | − |
| F163 | 6.25 | 6.25 | 1.56 | − | − | Ser (AGC) | − |
| F120 | 6.25 | 6.25 | 1.56 | − | − | − | − |
| B146, F112 | 6.25 | 6.25 | 3.13 | − | − | Ser (AGC) | − |
| 103, 118, B3, and B16 | 12.5 | 6.25 | 3.13 | − | − | − | − |
| B214 | 50 | 12.5 | 3.13 | − | − | Arg (CGC) | − |
| F51 | 50 | 25 | 25 | − | Lys (AAA) | Arg (AGA) | − |
| 139 | 50 | 25 | 25 | Arg (AGA) | − | Ile (ATC) | − |
| 102 | 100 | 25 | 12.5 | Asn (AAT) | − | Ile (ATC) | − |
| F219 | 100 | 50 | 50 | Arg (AGA) | − | Ile (ATC) | − |
| D61 | 100 | 100 | 50 | Ile (ATT) | − | Ile (ATT) | − |
| F121 | 200 | 50 | 50 | − | Gly (GGA) | Ile (ATT) | − |
| B159 and F159 | 200 | 100 | 50 | Ile (ATT) | − | Ile (ATC) | − |
| F77 | 200 | 200 | 100 | Ile (ATT) | − | Ile (ATC) | − |
| B452 | 200 | 400 | 50 | Ile (ATT) | − | Ile (ATT) | Ala (GCA) |
| F185 and B281 | 400 | 100 | 100 | Arg (AGA) | − | Ile (ATC) | − |
| B242 | 400 | 100 | 100 | Arg (AGA) | − | Ile (ATT) | − |
| B17 | 400 | 100 | 100 | Ile (ATT) | − | Ile (ATC) | − |
| 114 | 400 | 200 | 100 | Ile (ATT) | − | Ile (ATT) | − |
| F32 | 400 | 200 | 100 | Ile (ATT) | − | Ile (ATC) | − |
| B150 | 400 | 400 | 100 | Ile (ATT) | − | Ile (ATC) | − |
| F192 | 400 | 400 | 100 | Ile (ATT) | − | Ile (ATT) | − |
a
NFLX, norfloxacin; OFLX, ofloxacin; CPFX, ciprofloxacin.
b
−; identical to type strain.