KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
|
| ||
| Bacterial and virus strains | ||
|
| ||
| AAV9.Ef1a.fDIO.GCaMP6f | (Menegas et al.)49 | Addgene #118273 |
| AAV9.CAG.FLEX.TdTomato | (Oh et al.)50 | Addgene #51503 |
| AAV5-EF1a-DIO-hChR2(H134R)-eYFP-WPRE-HGHpA | Karl Deisseroth | Addgene #20298 |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Picrotoxin | Hello Bio | HB0506 |
| CGP 55845 | Tocris | 1248 |
| SST 14 | Bachem | H-1490 |
| Cyclosomatostatin (cyclo-SST) | Abcam | ab141211 |
| Octreotide Acetate | Sigma Aldrich | PHR 1880 |
| Tetrodotoxin (TTX) | Abcam | ab120054 |
| 2,2,2-Tribromoethanol (Avertin) | Thermo Fisher Scientific | A18706.14 |
| Isoflurane | Piramal Pharma Limited | NDC: 66794–013-25 |
| Ketoprofen (100 mg/ml injectable solution) | Manufacturer: Zoetis Supplier: Midwest Veterinary Supply |
275.22120.3 |
| Bupivacaine HCl | Manufacturer: Hospira Supplier: Midwest Veterinary Supply | 191.26505.3 |
| 4% Paraformaldehyde | Thermo Fisher Scientific | J19943.K2 |
| Kynurenic acid | Sigma | K3376 |
| Immu-Mount | Thermo Fisher Scientific | 9990402 |
| DAPI Fluoromount-G | Southern Biotech | 0100–20 |
|
| ||
| Deposited data | ||
|
| ||
| Code and Data | This study | Dryad:https://datadryad.org/stash/share/ouwbAlBUHoEafVxikaupvUOuhSn3wUHZYc096IYwM2c |
|
| ||
| Experimental models: Organisms/strains | ||
|
| ||
| C57BL/6J | Jackson Labs | #000664 |
| SST-IRES-Cre+/- | Jackson Labs | #013044 |
| VIP::Cre | Jackson Labs | #010908 |
| SST::Flp | Jackson Labs | #028579 |
| Df(16)A+/- | (Stark et al.)51 | N/A |
|
| ||
| Software and algorithms | ||
|
| ||
| Clampex (Clampfit and pClamp 11) | Molecular Devices | https://www.moleculardevices.com/ |
| Biorender | Biorender | https://www.biorender.com/ |
| Prism | Graphpad | https://www.graphpad.com/ |
| MATLAB | The Math Works, Inc. | https://www.mathworks.com/products/matlab.html |
| Python | (Van Rossum)52 | N/A |
| Deep Lab Cut | (Mathis et al.)53 | https://github.com/DeepLabCut/DeepLabCut |
|
| ||
| Other | ||
|
| ||
| Vibrating Microtome VF-300–0Z | Precisionary Instruments | N/A |
| Leica Vibratome VS. 1200 | Leica | N/A |
| 7000 smz2 Vibratome | Campden Instruments | N/A |
| Narishige PC-100 Puller | Narishige Group | N/A |
| Borosilicate Glass Capillaries | Warner Instruments | 64–0772 |
| 470-nm LED pE-100 | Cool LED | pE-100 |
| Contemporary Ortho-Jet Powder (Bilateral Cannula) | Lang Dental | Ref. 1530 |
| Ortho-Jet Liquid (Bilateral Cannula) | Lang Dental | Ref. 1304 |
| Dental Cement (Fiber Photometry) | Unifast Trad | N/A |
| Vetbond | 3M | 1469Sb |
| ICV Bilateral Internal Cannula | P1 Technologies | 8IC235ISPCXC |
| ICV Bilateral Guide Cannula | P1 Technologies | 8IC235G10XXC |
| ICV Bilateral Dummy Cannula | P1 Technologies | 8IC235DCSPCC |
| Bilateral Cannula Dust Cap | P1 Technologies | 8K0000303DC1 |
| Stereotaxic frame (Bilateral Cannula/ Optogenetic Release): Drill and Microinjector Robot, Mouse/Rat | Stoelting | SKU: 51705 |
| Stereotaxic frame (Fiber Photometry) | Kopf Instruments | Model 942 |
| 1 μL Neuros syringe | Hamilton | 65458–01 |
| 5 μL Removable Needle Syringe | Hamilton | #7634–01 |
| Syringe Pump | Stoelting | #53311 |
| miniature screws | Antrin Miniature Specialities | AMS90/1B |
| Fiber Optic Cannula (200 μm core, 0.39 NA) | ThorLabs | CFMC12L05 |
| 473-nm Laser | Ready Lasers | MBL-III-473–100mW |
| Noise Eeater | ThorLabs | NEL01 |
| Kinematic Flourescence Filter Cube | ThorLabs | DFM1 |
| Dual-Edge Dichoric Mirror | Chroma | ZT488/561rpc |
| FC/PC Fiber Coupler | Thorlabs | PAF2-A4A |
| Fiber Patchcord (200 μm core, 0.39 NA) | ThorLabs | M72L05 |
| Fiberoptic Rotary Joint | Doric Lenses | FRJ_1×1_FC-FC |
| Mating Sleeve | Precision Fiber Products | SM-CS1140S |
| Dual-band Emission Filter | Chroma | ZET488/561m |
| Fiber Coupler | ThorLabs | PAF2S-11A |
| Anti-reflection-coated Fiber | ThorLabs | M200L02S-A |
| Spectrometer | Ocean Insight | QE Pro |
| BX63 Upright Microscope | Olympus | N/A |
| CMS GmbH CMOS fitted Microscope | Leica Microsystems | N/A |
| LSM 800 Confocal Microscope | Zeiss | N/A |
| Waveform Generator | Sanworks | Pulse Pal v2 |
| Blackfly S USB3 Camera | FLIR | N/A |