Figure 6: A subset of active reservoirs display strong links to HIV-specific CD4⁺ and CD8⁺ T cell responses.

(A) Global Uniform manifold approximation and projection (UMAP) for dimension reduction map of HIV-specific AIM⁺ CD4 T cells. 15 clusters were identified and regrouped in 6 superclusters. (B) Median proportions of each cluster, regrouped by superclusters. (C-D) Heat map showing correlations between (C) vRNA⁺ cluster frequencies and net magnitudes of AIM⁺ CD4⁺, regrouped by superclusters, or (D) vRNA⁺ cluster frequencies and net magnitudes of AIM⁺ CD8⁺ T cells. P values from Spearman test are shown, with significance underneath. * : p <0.05, ** p <0.01, *** p <0.001. (E-F) Correlations between the inducible or spontaneous vRNA⁺ reservoir and the magnitude of cells in (E) AIM⁺ HIV-specific CCR6⁺ CD4⁺ T supercluster, (F) AIM⁺ HIV-specific CXCR3⁺ CD4⁺ T supercluster. (G-H) Correlations between the inducible or spontaneous p24⁺ reservoir and the magnitude of cells in (G) total AIM⁺ HIV-specific CD4⁺, and (H) total AIM⁺ HIV-specific CD8⁺ T cells. Spearman tests were performed. R and p values are shown. (IJ) Histogram comparing median HIV-specific AIM⁺ (I) CD4 and (J) CD8 T responses in people where p24⁺ cells were detectable in peripheral blood (n=5) or were not (n=11). Mann-Whitney tests are shown above. Error bars indicate the interquartile range. N=16 (1 participant had <5 vRNA⁺ cells, therefore could not be phenotyped).