Extended Data Figure 4. Single-molecule RNA in situ hybridization validation of selected differentially expressed genes across all cell types of dup15q and control cortical samples.

a) Validation of enrichment of UBE3A transcript in neurons of the temporal cortex. b) Validation of enrichment of GABRG3 transcript in neurons of the temporal cortex. c) Validation of PLXNA4 transcript downregulation in neurons of the temporal cortex. (n=3 cortical samples from both dup15q and controls. Statistics represents counts from 7 field. Scale bar = 100μm).