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A-F) WT IMMs were treated with LPS for 20 min, then Golgin-97, a trans-Golgi network marker (green), phospho-MARCKS (red) and the nuclei (blue) were visualized using specific antibodies.
C, G) Images of the same samples merged for analysis of colocalization of MARCKS and the trans-Golgi network.
D, H) The merge only panel is shown in yellow.
