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[Preprint]. 2023 Jul 10:rs.3.rs-3094036. [Version 1] doi: 10.21203/rs.3.rs-3094036/v1

Figure 4. LPS induces MARCKS expression through the TLR4 dependent pathway.

Figure 4

A, B) WT IMMs and ΔTLR4 IMMs were treated with LPS for 6 hours or left unstimulated (indicated as “unstim” in this and subsequent panels). TNF and MARCKS mRNA levels were measured using real-time PCR. Data are representative of two independent experiments and shown as mean ± SEM: ***p<0.005, **** p< 0.0001 (one-way ANOVA).

C) ΔTLR4 IMMs were treated with LPS for the indicated times. MARCKS protein expression was detected by western blot and beta-actin was used as a loading control. The full-length blot image is included in the Supplementary Information file, Supplementary Figure 3.

D) Densitometric analysis of western blot results. MARCKS was normalized to beta-actin. Data are representative of 2 independent experiments, shown as mean ± SEM.