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. 2023 Feb 24;23(6):2867–2875. doi: 10.1007/s10238-023-01024-1

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Workflow of the ExoView analysis from EV isolation to sEV profiling. EVs were isolated from plasma by size exclusion chromatography and subjected to ExoView analysis using the EV-TETRA-C chip. The lower panel shows potential tetraspanin profiles when capturing sEVs with anti-CD81, which is transferrable to sEVs captured with anti-CD63 and anti-CD9 although with some adjustments. The Figure was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license. *SP-single positive, DP-double positive, TP-triple positive