Fig. 3.

Single vesicle analysis of SEC isolated plasma sEVs. a Tetraspanin fluorescent staining of sEVs captured by anti-CD63 (red), anti-CD81 (green) and anti-CD9 (blue), b average ACP* distribution in RA patients and healthy controls for each tetraspanin capture probe, c number of fluorescent particles/ml captured by each capture probe, d phenotypic ACP analysis of CD9 single positive sEVs, CD81 single positive sEVs, anti-CD9 captured CD81/CD9 double positive sEVs and anti-CD81 captured CD81/CD9 double positive sEVs. For the analysis in d we compared the sample mean of the two study phenotypes using Welch’s two sample t-test for unequal variance. *ACP-Average colocalization percent, NFP-number of fluorescent particles/ml