Figure 1.

Non-transferrin-bound iron levels in Hbb^th3/+ mice afer vamifeport treatment alone or in combination with a single blood transfusion. Representative scatter plots of individual values (with mean and standard deviation) showing the effects of transfusion, vehicle, and vamifeport treatment. Donor blood was collected (A) 1 day or (B) 15 days before the blood transfusion. Analysis was performed by using one-way analysis of variance with Dunnett’s multiple comparison test. Significant differences compared with the Hbb^th3/+ vehicle transfused group are indicated as: *P<0.05, **P<0.01, and ***P<0.001. NTBI: non-transferrinbound iron.