Figure 2.

Hematological parameters in Hbb^th3/+ and wild-type mice afer vamifeport treatment alone or in combination with repeated blood transfusions. (A) Effects of transfusion, vehicle, and vamifeport treatment on hemoglobin levels. Timing of blood transfusions are indicated by red arrows. The remaining figures are representative scatter plots of individual values (with mean and standard deviation) showing the effects of transfusion, vehicle, and vamifeport treatment at the end of the study on: (B) red blood cell counts, (C) hematocrit levels, (D) reticulocyte counts. WT: wild-type. Parameters over time were analyzed by using two-way analysis of variance (ANOVA), with repeated measures for time course effects (A) and end point parameters by using one-way analysis of variance (B, C and D) with Dunnett’s multiple comparison test. Significant differences compared with the Hbb^th3/+ vehicle non-transfused group (black) and vehicle-transfused group (purple) are indicated as: *P<0.05, **P<0.01, and ***P<0.001. In part (A), significant differences between the Hbb^th3/+ vehicle non-transfused and the vehicle-transfused, vamifeport-treated transfused, and vamifeport-treated non-transfused groups are indicated in black, dark blue, and light blue, respectively. Significant differences between the vamifeport-treated transfused group compared with the vamifeport-treated non-transfused group are indicated in red (Wilcoxon matched pairs signed-rank test).